Download Electrotransformation of Bacteria by Natalie Eynard, Justin Teissie PDF

By Natalie Eynard, Justin Teissie

Creating micro organism with changed genetic houses permits the categorical research of those microorganisms. Electrotransformation is a hugely effective and simple to use strategy to brought genetic fabric into bacterial cells, with using a robust electrical box. within the current guide, protocols for the transformation of approximately forty traces of micro organism are defined. Emphasis is given at the person severe procedural steps, because the useful info rely on the bacterial pressure lower than research. This presentation including the theoretical introductionary chapters, permits the person to change and adapt each one protocol to his/her personal experiments. Bacterial lines with relevance within the nutrition and biotechnology, in clinical and veterinary fields, agroindustry and environmental sciences are covered.

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Bergey's manual of systematic bacteriology in Williams & Wilkins, Baltimore. Xie, T. , L. Sun and T. Y. Tsong. 1990. Study of mechanisms of electric field-induced DNA transfection. I. DNA entry by surface binding and diffusion through membrane pores. Biophys. J. 58:13-19 Xie, T. , L. Sun, H. G. Zhao, J. A. Fuchs and T. Y. Tsong. 1992. Study of mechanisms of electric field-induced DNA transfection. IV. Effects of DNA topology on cell uptake and transfection efficiency. Biophys J. 63:1026-1031 21 22 NA TALJE EYNARD and JusTIN TEISSIE Xie, T.

And T. Y. Tsong. 1990. Study of mechanisms of electric fieldinduced DNA transfection. li. Transfeetion by low-amplitude, lowfrequency alternating electric fields. Biophys. J. 58:897-903 Xie, T. D. and T. Y. Tsong. 1992. Study of mechanisms of electric fieldinduced DNA transfection. III. Electric parameters and other conditions for effective transfection. Biophys J. 63:28-34 Yamamoto, N. and T. Takano. 1996. Isolation and characterization of a plasmid from Lactobacillus helveticus CP53. Biosei Biotechnol Biochem.

1992. Improved method for electroporation of Staphylococcus aureus. Fems Microbiol Lett. , V. Mancino and B. Birren. 1995. arge DNA molecules by electroporation. Nucleic Acids Res. 23:1990-1996 Shepard, B. D. and M. S. Gilmore. 1995. Electroporation and efficient transformation of Enterococcus faecalis grown in high concentrations of glycine. Methods Mol Biol. , W. Förster, H. E. Jacob and R. Grigorova. 1983. Microbiological implications of electric field effects. VII. Stimulation of plasmid transformation of Bacillus cereus protoplasts by electric field pulses.

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