By W. C. Chan, Peter D. White
Within the years because the ebook of Atherton and Sheppard's quantity, the means of Fmoc solid-phase peptide synthesis has matured significantly and is now the traditional procedure for the regimen construction of peptides. the elemental difficulties extraordinary on the time of book of this past paintings have now been, for the main half, solved. consequently, innovators within the box have focussed their efforts to improve methodologies and chemistry for the synthesis of extra advanced constructions. the focal point of this new quantity is far broader, and covers not just the basic methods for the construction of linear peptides but additionally extra complicated ideas for getting ready cyclic, side-chain transformed, phospho- and glycopeptides. Many different tools additionally deserving awareness were integrated: convergent peptide synthesis; peptide-protein conjugation; chemoselective ligation; and chemoselective purification. The tricky practise of cysteine and methionine-containing peptides can be coated, in addition to tools for overcoming aggregation in the course of peptide chain meeting and a survey of accessible computerized instrumentation.
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Extra info for Fmoc Solid Phase Peptide Synthesis: A Practical Approach
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Furthermore, a wide range of groups is also available which can be selectively removed on the solid phase, thus enabling the selective modification of side-chains of individual residues within the peptide chain. These find application in the synthesis of cyclic peptides, phosphopeptides, and biotinylated peptides (see Chapter 6). Table 4 lists the most commonly used side-chain protecting groups, together with the conditions required for their removal; those recommended for routine use are highlighted.
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