By World Health Organization
This ebook is a brand new variation of a truly well known laboratory handbook released via WHO within the Nineteen Eighties. the hot variation is important as a result of new systems and applied sciences constructed because the prior variation that experience proved important to small laboratories in constructing international locations. those new systems and applied sciences were integrated within the proper sections of the guide, and a few out of date methods were changed by way of extra updated techniques.The handbook presents a pragmatic consultant to the secure and actual functionality of uncomplicated laboratory innovations. meant to be used through laboratory technicians operating in peripheral-level laboratories in constructing international locations, the ebook emphasizes basic, affordable methods that could yield actual effects the place assets, together with apparatus, are scarce and the weather is sizzling and humid.The publication is split into 3 components. the 1st describes the setting-up of a peripheral healthiness laboratory and common laboratory approaches, together with use of a microscope and laboratory balances, centrifugation, dimension and allotting of beverages, and cleansing, disinfection and sterilization of laboratory gear. tools of disposal of laboratory waste, dispatch of specimens to reference laboratories and laboratory security also are mentioned. the second one half describes concepts for interpreting varied specimens for helminths, protozoa, micro organism and fungi. recommendations for the training, fixation and marking of smears also are mentioned. The 3rd and ultimate half describes the exam of urine, cerebrospinal fluid and blood, together with innovations in line with immunological and serological ideas. for every approach, an inventory of fabrics and reagents is given, through an in depth description of the strategy and the result of microscopic exam. a variety of illustrations are used in the course of the publication to explain the several steps concerned. A precis of the reagents required for some of the ideas and their practise is equipped within the annex.
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Extra resources for Manual of Basic Techniques for a Health Laboratory
Differential counter Although a hand tally counter can be used, a differential counter saves time. Photometer or colorimeter It is necessary to have a photometer or colorimeter for blood chemistry tests and for accurate determination of haemoglobin levels. Battery-powered models are commercially available. 1. 3. 3. 2. 2 Additional items Autoclave If the laboratory is in a hospital, the hospital sterilization service can be used. 5): — a small autoclave (electric or heated by an oil stove or with butane gas) — a pressure cooker.
34 A demineralizer Quality control Apparatus with a control dial The dial registers the resistivity of the water resulting from the presence of ions. The more complete the demineralization, the higher the electrical resistivity of the water. 1. Check that the control system is fitted with a battery in good working order. 2. To check that the battery is charged, press the button marked “zero test”; the needle on the dial should swing to zero (Fig. 35(a)). 3. Let water flow into the cartridge. 4.
1 Registration of specimens All specimens must be registered and given numbers when they arrive at the laboratory and the results of all investigations must be recorded. This will: — prevent the specimens from getting mixed up; — make it possible to look up a result; — make the results available for the promotion of public health. 2. Setting up a peripheral health laboratory 47 The laboratory should have: — examination request forms that accompany the specimens; — a register for recording details concerning the specimens and the results obtained; — monthly report forms.