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Homozygote and heterozygote PCR products could be readily distinguished using a mixture of ddTTP and ddCTP, although primer extensions containing low amounts of the minor allele could not be fully resolved. e. A. C. R. L. P. SMIRNOV completely resolved peaks corresponding to ddC (+273 Daltons) and biotinylated ddU (+666 Daltons) extensions (figure 6). A plot of the measured allele ratios (by peak areas), vs. 2. This reflects the slightly greater incorporation rate of biotinylated ddUTP to ddCTP (figure 7).

A. C. R. L. P. SMIRNOV throughput MALDI-TOF, and associated robotic systems to provide greater throughput. 8. REFERENCES 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. Cargill M, Alshuler D, Ireland J, Sklar P, Ardlie K, Patil N, Lane CR, Lim EP, Kalyanaraman N, Nemesh j, Ziaugra L, Friedland L, Rolfe A, Warrington J, Lipshutz R, Daley GQ, Lander ES. Nature Genetics 22: 231, 1999 van Rensburg SJ, Potocnik FCV, deVilliers JNP, Kotze MJ, Taljaard JJP. Annals of New York Academy of Science 903: 200, 2000 Lutz CT, Foster PA, Noll WW, Voelkerding KV, Press RD, McGlennen RC, Kirschbaum NE.

5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. Cargill M, Alshuler D, Ireland J, Sklar P, Ardlie K, Patil N, Lane CR, Lim EP, Kalyanaraman N, Nemesh j, Ziaugra L, Friedland L, Rolfe A, Warrington J, Lipshutz R, Daley GQ, Lander ES. Nature Genetics 22: 231, 1999 van Rensburg SJ, Potocnik FCV, deVilliers JNP, Kotze MJ, Taljaard JJP. Annals of New York Academy of Science 903: 200, 2000 Lutz CT, Foster PA, Noll WW, Voelkerding KV, Press RD, McGlennen RC, Kirschbaum NE. Clinical Chemistry 44: 1146, 1998 Hoogendoorn B, Owen MJ, Oefner PJ, Williams N, Austin J, O’Donovan MC.

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