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By Robert R Harr

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Eighty WBCs are counted in the four large corner squares of a Neubauer hemacytometer. The dilution is 1:100. What is the total WBC count? A. 0 × 109/L B. 0 × 109/L C. 0 × 109/L D. 0 × 109/L Hematology/Calculate/Cell count/2 3. A manual RBC count is performed on a pleural Answers to Questions 1–3 1. 7 × 109/L where total WBC = WBCs × 109/L, 100 is the number of WBCs counted in the differential, and 126 is the sum of NRBCs plus WBCs counted in the differential. 2. 0 × 109/L 3. 0 × 109/L fluid. The RBC count in the large center square of the Neubauer hemacytometer is 125, and the dilution is 1:200.

What is the basic hematological defect seen in patients with thalassemia major? A. DNA synthetic defect B. Hgb structure C. β-Chain synthesis D. Hgb phosphorylation Hematology/Apply knowledge of fundamental biological characteristics/Hemoglobinopathy/1 9. Which of the following is the primary Hgb in patients with thalassemia major? A. Hgb D B. Hgb A C. Hgb C D. Hgb F Hematology/Correlate clinical and laboratory diseases/ Hemoglobin/Hemoglobinopathy/2 10. 0 × 1012/L. What is the morphological classification of this anemia?

Decreased serum iron concentration C. Ringed sideroblasts D. Pappenheimer bodies Hematology/Correlate laboratory data to recognize health and disease states/2 15 Answers to Questions 7–12 7. D Sideroblastic anemia has a decreased red cell protoporphyrin. The defect in sideroblastic anemia involves ineffective erythropoiesis. The failure to produce RBC protoporphyrin occurs because the nonheme iron is trapped in the mitochondria and is unavailable to be recycled. 8. C In thalassemia major, there is little or no production of the β-chain, resulting in severely depressed or no synthesis of Hgb A.

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