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Gen. Physiol. 44, 169 (1960). CHAPTER 3 Carbohydrate Transport in Bacterial Cells* Saul Roseman I. Introduction II. Modes of Transport A. General Comments B. Passive Diffusion C. Transport across Cell Membranes D. Transport of Sugars by Bacterial Cells E. Approaches to the Mechanism of Carbohydrate Transport III. The Phosphotransferase System A. General Comments B. Proteins of the Phosphotransferase System C. Role of the Phosphotransferase System in Sugar Transport D. Occurrence of the Phosphotransferase System E.

Or, to put it more exactly, that the constants in Fig. 1 of Chapter 1, k = k- k S = k- S , etc. In fact, it has rarely been shown that a process which resembles facilitated diffusion in all respects achieves an equilibrium state where internal and external concentrations are precisely the same. Perhaps this is not too surprising. The internal milieu certainly differs markedly from the external solu­ tion, and conceivably these differences could affect some of the kinetic constants. The characteristic features of the active transport process are identical with those of facilitated diffusion with only one key difference.

Physiol. 46, 343 (1962). 30. Μ. H. Zarlengo and S. G. Schultz, Biochim. Biophys. Acta 126, 308 (1966). 31. W. Epstein and S. G. Schultz, J. Gen. Physiol. 49, 469 (1966). A. Rothstein, unpublished observations (1969). 32. E. J. Conway, H. Ryan, and E. Carton, Biochem. J. 58, 158 (1954). 33. J. Barber, Biochim. Biophys. Acta 150, 730 (1968). 34. F. M. Harold and J. R. Baarda, Biochem. J. 6, 3107 (1967). 35. R. Damadian and A. K. Solomon, Science 145, 1327 (1964). 36. H. L. Ennis and M. Lubin, Biochim.

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