By Juan Hong Juan A. Asenjo
content material: Protein free up from chemically permeabilized Escherichia coli / David J. Hettwer and Henry Y. Wang --
dependent and easy versions of enzymatic lysis and disruption of yeast cells / J.B. Hunter and J.A. Asenjo --
twin hollow-fiber bioreactor for cardio whole-cell immobilization / Ho Nam Chang, Bong Hyun Chung, and In Ho Kim --
A membrane reactor for simultaneous construction of anaerobic single-cell protein and methane / R.K. Finn and E. Ercoli --
Membrane approaches within the separation, purification, and focus of bioactive compounds from fermentation broths / Enrico Drioli --
Liquid emulsion membranes and their functions in biochemical separations / M.P. Thien, T.A. Hatton, and D.I.C. Wang --
Use of aqueous two-phase platforms for restoration and purification in biotechnology / Bo Mattiasson and Rajni Kaul --
restoration of proteins from polyethylene glycol-water answer by means of salt partition / G.B. Dove and G. Mitra --
Modeling of precipitation phenomena in protein restoration / C.E. Glatz and R.R. Fisher --
procedure concerns for scale-up of liquid chromatography and electrophoresis / S.R. Rudge and M.R. Ladisch --
Mathematical modeling of bioproduct adsorption utilizing immobilized affinity adsorbents / Somesh C. Nigam and Henry Y. Wang --
High-resolution, high-yield continuous-flow electrophoresis / William A. Gobie and Cornelius F. Ivory --
Scale-up of isoelectric focusing / Milan Bier --
Large-scale gel chromatography : evaluation of software for purification of protein items from microbial resources / James J. Kelley, George Y. Wang, and Henry Y. Wang --
Electron paramagnetic resonance spectroscopy stories of immobilized monoclonal antibody constitution and serve as / Erik J. Fernandez, Forrest B. Fernandez, Roger B. Jagoda, and Douglas S. Clark.
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Content material: Protein liberate from chemically permeabilized Escherichia coli / David J. Hettwer and Henry Y. Wang -- based and easy types of enzymatic lysis and disruption of yeast cells / J. B. Hunter and J. A. Asenjo -- twin hollow-fiber bioreactor for cardio whole-cell immobilization / Ho Nam Chang, Bong Hyun Chung, and In Ho Kim -- A membrane reactor for simultaneous creation of anaerobic single-cell protein and methane / R.
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Extra resources for Separation, Recovery, and Purification in Biotechnology. Recent Advances and Mathematical Modeling
Niger growth. It was observed that the flow rate decreased gradually to zero meaning that the pumping head of a p e r i t a l t i c pump was not s u f f i c i e n t to overcome the flow resistance exerted by the growing fungi. Thus the continu ous operation of the reactor was not f e a s i b l e . It i s suggested that the control of the c e l l growth be needed after a growth period by switching the culture to a nitrogen d e f i c i e n t medium or by some other means. Production of rifamycin Β by N.
The cross section of a dual hollow f i b e r unit i s shown i n Figure 1 wherein microbial c e l l s are supposed to grow i n the r e s t r i c t e d i n t e r s t i t i a l space between the two f i b e r walls. The detailed dimensions of the reactor are shown i n Figure 2. 12 cm^. The c e l l inoculum port was covered with rubber through which inoculation could be made with a syringe needle. Reactor operation. The polypropylene hollow f i b e r s i n the reactor were prewetted prior to inoculation with r e c i r c u l a t i o n of 50% ethanol and s t e r i l i z e d chemically with 5% formalin solution.
In a l l the f i b e r s the c e l l s grew well and appeared uniform along the f i b e r s , but the s i l i c o n e tubes were expan ded and the polypropylene tubes were contracted. Figure 6(a) i s the photograph of an empty s i l i c o n e tube and Figure 6(b) shows the cross section of the f i b e r after 15 days of the A_. niger growth. It was observed that the flow rate decreased gradually to zero meaning that the pumping head of a p e r i t a l t i c pump was not s u f f i c i e n t to overcome the flow resistance exerted by the growing fungi.